The third fraction is comprised of prostatic fluid and is normally clear. After a brief period of rest, the dog will ejaculate the third seminal fraction.The pre-sperm fraction arises from the prostate and urethral glands, and is believed to cleanse the urethra of contaminants (urine, bacteria and cellular debris) prior to ejaculation The second or sperm-rich fraction originates in the cauda of the epididymis where spermatozoa are stored. The first or pre-sperm fraction is of prostatic origin and is clear. During this period, the first and second seminal fractions are ejaculated. With the onset of ejaculation, dog will thrust vigorously for several minutes.The collector uses his or her thumb and finger to form a ring proximal to the bulbis glandis and pressure is applied to the penis circumferentially, thus simulating the copulatory lock or "tie.".The collecting cone covers the penis to the level of the bulbis glandis. Concurrent with prepucial positioning, the collecting cone is introduced over the engorging penis.Failure to do so may result in a "paper cut" type of injury to the penis. It is important to roll over the edge of the cone such that the edge of the cone does not come into direct contact with penis. The collection process may then become painful to the dog as the skin of the prepuce tightens over the engorged bulbis glandis. Failure to reposition the prepuce at the correct time may result in an inability to slide the prepuce proximally over the bulbis glandis. As soon as erection begins, the prepuce is moved proximally such that the entire prepuce is proximal to the exposed bulbus glandis. Other males require brisk massage of the bulbus glandis through the prepuce to elicit erection. Some males will achieve erection without any manual manipulation. It is important to be aware of the onset of penile erection.During collection, the dog should be observed for ease of achieving an erection, presence of a normal erection, normal thrusting behavior and normal pulsation associated with ejaculation and prostatic fluid emission. Signs of readiness include salivation and "chomping" of his teeth. ![]() He may sniff or lick her external genetalia or rear limbs.
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